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Correlative microscopy of living cells

Ao Univ. Prof. Dr. Irene Lichtscheidl
University of Vienna,
Core Facility Cell Imaging and Ultrastructure Research
Althanstrasse 14, A-1090 Vienna, Austria

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Abstract:
Microscopy is a major tool to investigate cells and their contents. Bright field and contrast methods in light microscopy such as differential interference contrast, phase contrast, dark field and polarisation resolvesturctures as small as 200µm. In addition, submicroscopical structures are visualised in fluorescence and confocal laser scan microscopy as well as by video-enhanced contrast and ultraviolet microscopy. For further resolution, analysis in the electron microscope is required, which needs, however, fixing and embedding of samples. In correlative microscopy, we aim at observing the dynamic behavior of living cells and their organelles in the light microscope in as much detail as possible, and further preparethem in such a way for electron microscopy, that the same structures become resolved in their ultrastructure.

Keywords:
light microscopy, fluorescence microscopy, confocal laser scanning microscopy, electron microscopy, ultrastructure

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